Pedri, 18, has made 40 appearances, including 26 in La Liga, since joining Barca from Las Palmas last summer.
Spain coach Luis Enrique had monitored the Under-21 player in recent months and has named him in his 24-man squad.
⚠️ OFICIAL | Estos son los jugadores convocados por Luis de la Fuente para disputar la Fase de Grupos del Europeo Sub-21.
🆚 El combinado nacional, encuadrado dentro Grupo 🅱️, se enfrentará a Italia, Eslovenia y República Checa.
— Selección Española de Fútbol (@SeFutbol) March 15, 2021
Of the 24 players called up by Luis Enrique, nine of them are 23 and under — with Pedri being the youngest.
Manchester United goalkeeper David de Gea has kept his place in the squad. Barcelona left-back Jordi Alba, Liverpool midfielder Thiago Alcantara and Leeds United defender Diego Llorente return to the fold after missing previous call-ups.
Spain open their World Cup qualifying campaign against Greece in Granada on March 25 before travelling to Tbilisi to face Georgia three days later. La Roja host Kosovo in Seville on March 31.
Squad in full
Defenders: Pedro Porro (Sporting), Eric Garcia (Manchester City), Sergio Ramos (Real Madrid), Diego Llorente (Leeds United), Inigo Martinez (Athletic Bilbao), Jordi Alba (Barcelona), Jose Luis Gaya (Valencia)
Midfielders: Sergio Busquets (Barcelona), Rodrigo (Man City), Thiago Alcantara (Liverpool), Pedri (Barcelona), Marcos Llorente (Atletico Madrid), Sergio Canales (Real Betis), Koke (Atletico Madrid), Fabian Ruiz (Napoli)